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The palm oil industry in Indonesia produces a large amount of liquid waste, Palm Oil Mill Effluent (POME), which poses environmental risks if improperly managed. This study evaluates the potential of POME as a renewable energy source in bio-batteries. POME was tested at three concentrations: 60%, 80%, and 100%, with copper (Cu) and magnesium (Mg) electrodes to measure voltage, current, and energy conversion efficiency. The bio-battery with 80% POME concentration showed the highest performance, generating 1.874 V, 60 mA, and an efficiency of 92%. The 60% POME bio-battery produced 1.753 V and 45 mA with 85% efficiency, while 100% POME produced 1.759 V, 50 mA, and 88% efficiency. Over a 30-day testing period, the 80% POME bio-battery demonstrated excellent stability, indicating potential for long-term renewable energy use. The study concludes that 80% POME concentration is optimal for efficient energy conversion and offers a promising environmentally friendly solution for generating renewable energy from palm oil mill waste.